Target: CENPA
Conjugate: Unconjugated
Product Type: Monoclonal
Clone Number: 5A7-2E11
Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
Swiss-Prot: P49450
Purification: Protein G Purified
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~18kDa. Recognizes Human CENP-A.
Cellular Localization: Nucleus,Chromosome,Centromere,Kinetochore
Scientific Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
References: 1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318.2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188.3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223.4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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