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Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: IKKβ (inhibitor of nuclear factor kappa B kinase subunit beta), also known as IKK2, is a protein subunit encoded by the IKβKβ (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta) gene. IKKβ activates NF-κB (nuclear factor kappa-light chain enhancer of activated B cells) signaling by phosphorylating IκBα (inhibitor of NF-κB) and leading to its degradation, allowing the release and translocation of NF-κB to the nucleus. NF-κB is a family of transcription factors that regulate gene expression in a variety of tissue types, and regulate the immune system, cell proliferation, differentiation, and apoptosis. Mutations in IKKB result in defective NF-κB signaling, which has been linked to immunological disorders and cancer. The use of inhibitors towards this protein, such as SAR113945, can become a therapeutic option in autoimmune disorders and cancer.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ IKKβ Kinase Assay Kit is designed to measure IKKβ (inhibitor of nuclear factor kappa B kinase subunit beta) serine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified IKKβ, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Uniprot: O14920
Warnings: Avoid freezee/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Schmid J. and Birbach A., 2008 Cytokine Growth Factor Rev 19(2) :157-65.
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