Products from BPS Bioscience require a minimum order value above 400€Application: Study enzyme kinetics and screening small molecule inhibitors for drug discovery and HTS applications.
Contraindications: The Chemi-Verse™ PARP7 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.
Description: The Chemi-Verse™ PARP7 Assay Kit is an ELISA-type chemiluminescent assay designed to measure PARP7 enzymatic activity for screening and profiling applications. PARP7 catalyzes the NAD-dependent ADP-ribosylation of histones. This 96-well or 384-well format assay kit contains sufficient amounts of purified PARP7 enzyme (amino-acids 400- 657), histone mixture, and PARP assay buffer for 100 or 400 enzyme reactions. The Chemi-Verse™ PARP7 Assay Kit takes advantage of a highly specific ADP-ribose binding reagent. First, histone proteins are coated on a 384-well plate. Next, an NAD
+ substrate is incubated with the PARP7 enzyme in an optimized assay buffer. Finally, the plate is treated with the ADP-ribose specific binding reagent and secondary HRP-conjugated antibody, followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Figure 1. Chemi-Verse™ PARP7 chemiluminescent assay schematic.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This 96-well format assay kit contains sufficient amounts of purified PARP7 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Uniprot: Q7Z3E1
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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