Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine- mediated signaling. This family includes four kinases: JAK1, JAK2, JAK3 and TYK2 (Tyrosine Kinase 2). Various studies have suggested that regulation of JAK activity is a promising approach to combat numerous diseases such as immune deficiency and cancer. Upon binding of a cytokine to its corresponding receptor, the receptor changes conformation and recruits two JAK molecules, which phosphorylate the receptor and activate a signal that results in STAT dimerization and translocation to the nucleus. This results in expression of pro-inflammatory cytokines and growth factors. For example, IL-12 and IL-23 receptor pathways require a TYK2/JAK2 heterodimer. TYK2 is also required for type interferon and IL-10 signaling. TYK2 has been closely linked to psoriasis, with individuals that have loss-of-function mutations being protected from this disorder. This points to the possibility of inhibiting TYK2 as a treatment option. Several TYK2 inhibitors have been developed for the treatment of psoriasis, hidradenitis suppurative, IBD (inflammatory bowel disease), dermatomyositis and SLE (systemic lupus erythematosus). Deucravacitinib, an allosteric inhibitor that binds to the pseudokinase domain, has been approved in 2022 for the treatment of plaque psoriasis. This approval indicates the potential of targeting TYK2 to treat inflammatory disorders.
The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-VerseTM TYK2 Kinase Assay Kit is designed to measure TYK2 (Tyrosine Kinase 2) tyrosine kinase activity for screening and profiling applications using ADP-GloTM as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified TYK2, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability:
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: P29597
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Rusinol L. and Puig L., 2023 Int J Mol Sci 24(4) :3391.
Morand E., et al., 2024 Nature Reviews Rheumatology 20 :232-240.
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