Source: Chickens were immunized with EQKLISEEDL (C-MYC) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Formulation: Provided in phosphate buffered saline with 0.09% sodium azide.
References: 1. Mata-Fink J, Kriegsman B, Yu HX, Zhu H, Hanson MC, Irvine DJ and KD Wittrup. 2013. Rapid Conformational Epitope Mapping of Anti-gp120 Antibodies with a Designed Mutant Panel Displayed on Yeast. J Mol Biol. 425:444-56.
2. Van Deventer JA, Wittrup KD. 2014.Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation. Methods Mol Biol. 1131:151-81.
3. Wang, S., Mata-Fink J., Kriegsman B, Hanson, M, Irvien DJ, Eisen HN, Burtn DR, Wittrup, KD, Kardar M, Chakraborty, AK. 2015. Manipulating the Selection Forces during Affinity Maturation to Generate Cross-Reactive HIV Antibodies.Cell 160(4):785-97.
4. Heimer, BW, Tam, BE and HD Sikes. 2015. Characterization and directed evolution of a methyl-binding protein for high-sensitivity DNA methylation analysis. Protein Eng Des Sel 28:543-551.
5. de Picciotto S, Dickson PM, Traxlmayr MW, Marques BS, Socher E, Zhao S1, Cheung S, Kiefer JD, Wand AJ, Griffith LG, Imperiali B, Wittrup KD. 2016. Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.J Mol Biol. 428(20):4228-4241.
6. Traxlmayr MW, Kiefer JD, Srinivas RR, Lobner E, Tisdale AW, Mehta NK, Yang NJ, Tidor B, Wittrup KD. 2016. Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library. J Biol Chem 291 (43): 22496-22508.
7. Kauke, MJ, MW, Traxlmayr, MW, Parker, JA, Kiefer, JD, Knihtila, R, McGee, J, Verdine, G, Mattos, C and KD Wittrup. 2017. An engineered protein antagonist of K-Ras/B-Raf interaction. Sci. Rep. 7: 5831.
8. Angelini A, Miyabe Y, Newsted D, Kwan BH, Miyabe C, Kelly RL, Jamy MN, Luster AD, Wittrup KD. Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis. 2018. Nat Commun. 13;9(1):1461. doi: 10.1038/s41467-018-03687-x
2. -Karlsson,A, Protein and peptide engineering for improving therapies for applications in Human health.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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