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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: CHK1 is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint at the G2/M phase transition in response to DNA damage. Activated CHK1 triggers a pleiotropic cellular response via phosphorylation of downstream effectors. For example, Chk1 activates and phosphorylates Wee1 kinase and regulates Cdc25C in response to DNA damage in G2 phase. Its activity is highly controlled at the G2/M phase transition.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The CHK1 Kinase Assay Kit is designed to measure CHK1 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant CHK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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