Application Note: Anti-Chloramphenicol-Acetyl Transferase Antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
Concentration Value: 85 mg/mL
ELISA Dilution: 1:5,000 - 1:25,000
Immunohistochemistry Dilution: User Optimized
Western Blot Dilution: 1:100 - 1:1,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near a 30-60 of bacterial chloramphenicol acetyl transferase (CAT). This antibody identifies recombinant CAT as a predominant band of 26 kDa in eukaryotic cells transfected with a plasmid bearing the CAT gene.
Physical State: Lyophilized
Purity and Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified chloramphenicol acetyl transferase (CAT). Cross reactivity against chloramphenicol acetyl transferase from other tissues and species may occur but have not been specifically determined.
Background: Chloramphenicol acetyltransferase enzyme is an effector of chloramphenicol resistance in bacteria.
Low Endotoxin: No
Other: User Optimized
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