Formulation: A solution in water
Purity: ≥80% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Histone H4 is one of four core histone proteins that is involved in the organization of DNA into chromatin.{18136} Histones are subject to a variety of post-translational modifications (PTMs), such as methylation, acetylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Histone H4 can be citrullinated at the arginine residue at position 3 (H4R3) by peptidyl arginine deiminase 4 (PAD4; Item No. 10500).{46198} Citrullination of H4R3 increases in U2OS cells following induction of DNA damage by adriamycin (doxorubicin; Item No. 15007) and is localized near fragmented nuclei.{46406} H4R3 citrullination is associated with smaller tumor size and inversely associated with p53 levels in tumor tissue samples derived from patients with non-small cell lung cancer (NSCLC). Citrullinated histone H4 is present in neutrophil extracellular traps (NETs) generated by stimulation of granulocytes with phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) and can be recognized by autoantibodies present in the serum of patients with rheumatoid arthritis.{46407} This product contains purified histone H4 (human, recombinant; His-tagged) (Item No. 38257) that has been modified with PAD4, which is subsequently depleted by affinity chromatography.
English
Deutsch
