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Applications: Useful for the kinase assay kits and Western blotting.
Background: CK1ε is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1ε is a regulator of Yes-associated protein (YAP) transcription coactivator which is a key regulator of organ size and a candidate human oncogene. CK1ε is activated by CCK2R and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells (1). CKIε can phosphorylate topoisomerase (topo) IIalpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs (2).
Description: Full length recombinant human CK1ε, also known as CSNK1E, HCKIE, MGC10398, and CK1E. Genbank Accession NM_001894, with an N-terminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 72kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Genbank: NM_001894
Purity: ≥70%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles.
Tags: GST-Tag
Uniprot: P49674
Biosafety Level: Not applicable (BSL-1)
References: 1. von Blume, J., et al., EMBO J 2007;26(22):4619-33.
2. Grozav. A G, et al., Nucleic Acids Res. 2009;37(2):382-92.
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