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Encompassing Amino Acids: 544-976(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Tyr Peptide 6, Invitrogen) at room temperature for 1 hour.
Description: Human cKit (V560G) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine560 to glycine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_000222
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P10721
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.