Clone: N/A
Label: FITC
Background: FITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measuring the flourescence intensity at 520 nm (Emission)/490 nm (Excitation).
Concentration: 1 mg/ml
Source: Bovine skinWe certify that:a) The collagen fluorescein is of animal origin only.b) The collagen fluorescein was derived only from animals which were slaughtered in the United Statesin USDAinspected abattoirs where the animals were subjected to ante- and post mortem inspectionand the animals were fit for use in laboratory studies.c) The United States if free from foot and mouth disease and rinderpest.
Purity: >95%
References: 1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-7622. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-3073. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-2634. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-12635. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-3346. Baici, A., Cohen, G., Fehr, K. and Boni, A., Anal. Biochem., 108, 230, (1980).
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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