Background: Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type I 100%, rat collagen II, III, IV and V <0.1%; rat elastin and fibronectin <0.1% ( RIA at 1:200 dilution).
Positive Control: Rat skin
Immunogen: Purified collagen type I from rat skin
Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration: app. 1 mg/ml
References: 1. Al Adnani M.S. (1989) Differential immunohistochemical localization of cytokeratins and collagen types I and III in experimentally-induced cirrhosis. J. Pathol. 159, 151-158. 2. Vialle-Presles M.J., Hartmann D.J., Franc S., Herbage D (1989) Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat. Histochemistry 91, 177-184. 3. Breen E., Falco V.M., Absher M., Cutroneo K.R. (1990) Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs. J. Biol. Chem. 265, 6286-6290. 4. Bouvier M., Couble M.L., Hartmann D.J., Magloire H. (1991) Isolation and characterization of rat alveolar bone cells. Cell Mol. Biol. 37, 509-517
UniProt: P02454
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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