Background: Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type II 100%, no crossreactivity with chicken collagen type I and III, no crossreactivity with human, mouse and rat collagen type II..
Positive Control: Chicken cartilage.
Immunogen: Purified collagen type II from fetal chicken cartilage.
Purification Method: affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 1 mg/ml.
References: 1. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006. 2. Mallein-Gerin F., Ruggiero F., Garrone R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204-211. 3. Anselme K., Lanel B., Gentil C., Hardouin P., Marie P.J., Sigot-Luizard M.F. (1994) Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 4, 113-123. 4. Rousseau J.C., Farjanel J., Boutillon M.M., Hartmann D.J., van der Rest M., Moradi-Ameli M. (1996) Processing of Type XI Collagen. J. Biol. Chem. 271, 23743-23748.
UniProt: P02460 (CO2A1_CHICK).
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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