Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type IV 100%; bovine collagen I, II, III, IX, XI and bovine fibronectin negative in ELISA at 1:100 dilution (< 0.1%).
Positive Control: Bovine skin or lens capsule.
Immunogen: Purified collagen type IV from bovine lens capsule.
Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 1 mg/ml.
References: 1. Guerret S, Govignon E, Hartmann DJ, Ronfard V. (2003) Long-term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Repair Regen. Jan-Feb;11(1):35-45. 2. Vitellaro-Zuccarello L., Garbelli R., Dal Pozzo Rossi V. (1992) Immunocytochemical localization of collagen types I, III, IV, and fibronectin in the human dermis. Cell Tissue Res. 268, 505-511. 3. Lannes-Vieira J., Dardenne M., Savino W. (1991) Extracellular matrix components of the mouse thymus microenvironment : Ontogenetic studies and modulation by glucocorticoi•d hormones. J. Histochem. Cytochem. 39, 1539-1546.
UniProt: Q7SIB2 (CO4A1_BOVIN).
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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