Background: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100%, porcine collagens type I, II, and III <0.1%, porcine elastin, fibronectin and laminin < 0.1% (RIA at 1:5000 dilution).
Positive Control: Porcine skin or liver.
Concentration: app. 1 mg/ml.
Source: Rabbits were immunized with purified collagen type IV from porcine kidney tissue.
References: 1. Boucard N, Viton C, Agay D, Mari E, Roger T, Chancerelle Y, Domard A ( 2007) The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. Aug;28(24):3478-88.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.