Background: Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type V 100%, human collagen type I, III and IV <0.1%, human fibronectin and laminin <0.1% (RIA at 1:100 dilution).
Positive Control: Human placenta.
Immunogen: Purified collagen type V from human placenta.
Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 1 mg/ml.
References: 1. Laurent R., Nallet A., Obert L., Nicod L., Gindraux F. (2014) Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. Jun;15(2):267-75. 2. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 3. Chanut-Delalande H., Fichard A., Bernocco S., Garrone R., Hulmes D.J.S., Ruggiero F. (2001) Control of heterotypic fibril formation by collagen V is determined by chain stoichiometry. J. Biol. Chem. 276, 24352-24359. 4. Kleman J.P., Hartmann D.J., Ramirez F., Van der Rest M. (1992) The human rhabdomyosarcoma cell line A204 lays down a highly insoluble matrix composed mainly of 1 type-XI and 2 type-V collagen chains. Eur. J. Biochem. 210, 329-335.
UniProt: P20908 (CO5A1_HUMAN).
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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