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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The TtCsm6 RNase activity was determined using a fluorogenic substrate, with fluorescent signal measured over time.
Description: Recombinant T. thermophilus TtCsm6 (type III-A CRISPR-associated protein Csm6). This construct contains a C-terminal His-tag. TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that contains an N-terminal CARF domain and C-terminal HEPN domain.
Format: Aqueous buffer solution.
Formulation: 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 1 mM DTT, 150 mM imidazole, and 10% glycerol
Genbank: WP_011229148
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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