Application Note: Anti-Cyclin-A antibody has been tested by western blot and immunohistochemistry and is suitable for the detection by immunoblot, ELISA, IHC, and IP of human, rat and mouse Cyclin A. Researchers should determine optimal titers for applications that are not stated below.
Concentration Value: 80 mg/mL
ELISA Dilution: 1:5,000 - 1:20,000
Immunohistochemistry Dilution: 1:100
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Cyclin A was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 60 kDa Cyclin A protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A from other species may also occur.
Background: Anti-Cyclin A detects Cyclin A. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-Cyclin A Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Low Endotoxin: No
Other: User Optimized