Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, the antibody against 5-methylcytosine, the secondary HRP-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Svedruzic, Z.M., Curr Med Chem. 2008;15(1):92-106.Application Reference(s):
Shamma, A., et al. Mol Cell Biol. 2013 Aug;33(16):3113-24. doi: 10.1128/MCB.01597-12. ATM mediates pRB function to control DNMT1 protein stability and DNA methylation (2013)
English
Deutsch
