Background: The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements.Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies.Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450, in the 4-parameter analysis. It cannot be used to determine the concentration of a different type of dsRNA. The only proper standard for each specific application is the purified dsRNA under investigation.
Source: In vitro synthesized RNA (heteropolymer consisting of unmodified ribonucleotides)
Purity: Absorbance spectrum
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