Description: dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3'-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.
The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.