Application Note: Elastase antibody is suitable for ELISA and western blot. Usage in other immunoassays such as immunohistochemistry is expected but has not been determined. Optimal concentrations should be determined by researcher.
Concentration Value: 80 mg/mL
ELISA Dilution: 1:20,000 - 1:100,000
Immunohistochemistry Dilution: User Optimized
Western Blot Dilution: 1:2,000 - 1:10,000
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Immunogen: Elastase [Porcine Pancreas]
Physical State: Lyophilized
Purity and Specificity: Elastase antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity against Elastase from other tissues and species may occur but have not been specifically determined.
Background: Anti elastase antibody detects elastase enzyme. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine.
Low Endotoxin: No
Other: User Optimized