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Encompassing Amino Acids: 569-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Invitrogen Zlyte Peptide 1) at room temperature for 1 hour.
Description: Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.
Genbank: NM_004442
Host Cell Line: Sf9 cells
Storage Stability: >12 months at -80°C
Tags: N-terminal His-tag
Uniprot: P29323
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Macrae,M., et al., Cancer Cell 8 (2), 111-118 (2005).
2. Xu,H., et al., Am. J. Physiol. Renal Physiol. 288 (4), F855-F866 (2005).