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Encompassing Amino Acids: EPOR: 273-338, JAK2: 36-1132(end)
Description: Complex of Human EPOR, also known as Erythropoietin receptor, GenBank Accession No. NM_000121, a.a. 273-338 with N-terminal His-tag, MW=9 kDa, and JAK2, also known as Janus Kinase 2, with V617F, Y1007F and Y1008F mutations, GenBank Accession No. NM_004972, a.a. 36-1132(end), with N-terminal FLAG-tag, MW=128 kDa. The proteins were expressed as single fusion protein with a (GSSG)6 linker in a Baculovirus infected Sf9 cell expression system.
Formulation: 50 mM Tris-HCl pH 8.0, 500 mM NaCl, 10% glycerol, 3 mM DTT.
Genbank: EPOR: NM_000121, JAK2: NM_004972
Purity: 0,89
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: EPOR: N-terminal His-tag, JAK2: N-terminal FLAG-tag
Uniprot: EPOR: P19235, JAK2: O60674
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.
2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.
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