Clone: CS1-4 Cocktail.
Background: The antibody cocktail is positive for the latent membrane protein-1 (LMP1) of the Epstein-Barr Virus. It strongly reacts with EBV-infected lymphoblastoid cell lines and infected B-cell immunoblasts in infectious Mononucleose and atypical lymphoproliferations in immunosuppressed patients. It is positive in app. 25-30 % undifferentiated nasopharyngeal carcinomas and in 40-50% of Hodgkin's diseases. Due to the lack of LMP in Burkitt's disease the cocktail is unreactive with these cells. Epstein Barr Virus is a member of the herpes viridae family and is involved in the development of infectious mononucleosis. EBV infections are also discussed in connection with the induction of several tumours. Generally EBV is found in Burkitt's lymphoma and in nasopharyngeal carcinoma. Additionally it is found in 40-50 % of Hodgkin lymphoma and app. 20-30 % of anaplastic CD30+ large cell lymphoma. Hydrophilic C-terminal end of the 60 kDa late membrane protein 1 of Eppstein Barr Virus.
Immunogen: Fusion protein of beta-galactosidase and EBV-late membrane antigen.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Concentration: 50 µg/ml.
References: 1. Rowe M., Rowe D.T., Gregory C.D., Young L.S., Farrell P.J., Rupani H., et al. (1987) Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt's lymphoma. EMBO J. 6; 2743-2453. 2. 2. Harabuchi Y., Yamanaka N., Kataura A., Imai S., Kinoshita T., Mizuno F., et al. (1990) Epstein-Barr virus in nasal T-cell lymphomaas in patients with lethal midline granuloma. Lancet 335; 128-130. 3. Herbst H. Dallenbach F. Hummel M., Niedobietek G. Finn T., Young L.S., et al. (19919 Epstein-Barr virus DNA and latent gene products in Ki-1 (CD30)-positive anaplastic large cell lymphoma. 4. Murray P.G., Young L.S., Rowe M., and Crocker J. (1992) Immunohistochemical demonstration of the Epstein-Barr virus encoded latent membrane protein in paraffin sections of Hodgkin's disease. J. Pathol. 166; 1-5.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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