Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
Description: The biological characterization of exosomes requires in most cases the isolation of intact exosomes. In this sense, a large number of methods have been developed for the isolation of exosomes from biological fluids, among which are ultracentrifugation, chromatography, filtration, immunological separation and polymer-based precipitation2. Each one of these methods presents its advantages and disadvantages, being the duration of the method, the need to have specialized equipment, the volume of sample, the purity and the low recovery, some of the disadvantages that these methods present. The solution of precipitation of Exosomes (EPS) is framed within the methods of precipitation of exosomes based on polymers, and among its main advantages it stands out its simplicity, speed, reproducibility, the slight effect in isolated exosomes and the use of neutral pH. And thanks to its specific formulation, it limits some of the disadvantages of this type of methods such as the co-isolation of non-vesicular contaminants, including lipoproteins and the presence of polymer material that might not be compatible with the downstream analysis. 1. Yáñez-Mó M, Siljander P, Andreu Z, Bedina Zavec A, Borrás F, Buzas E et al. Biological properties of extracellular vesicles and their physiological functions. Journal of Extracellular Vesicles. 2015;4(1):27066. 2. Théry C, Amigorena S, Raposo G, Clayton A. Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids. Current Protocols in Cell Biology. 2006;.
English
Deutsch
