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Encompassing Amino Acids: 2-end (all components)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme was incubated with 2 µM histone mixture with 40 µM AdoMet in HMT buffer 2A in a 10 µl reaction for 60 min at RT. 5 µl of Anti-Rabbit AlphaLISA® acceptor beads (1:250) and 5 µl of Primary antibody 6 (1:50) were added and incubation continued for 30 min. Nickel Donor beads (1:125) were added (10 µl) and mixture was further incubated for 45-60 min at RT before A-counts were measured.
Description: Complex of human EZH1, GenBank Accession No. NM_001991, a.a. 2-end, with N-terminal His-tag, MW= 86 kDa; human EED, NM_003797, a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa; human SUZ12, NM_015355, a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, co-expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,10 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol.
Genbank: NM_001991 (EZH1), NM_003797(EED), NM_015355 (SUZ12)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag (EZH1, SUZ12), N-terminal FLAG-tag (EED)
Uniprot: Q15910
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Margueron R. et al. Mol. Cell 32, 4, 503-518, 2008.
2. Shen X. et al., Mol. Cell 32, 4, 491-502, 2008.
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