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Encompassing Amino Acids: 2 - 399 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction was monitored by measuring NADH production by following absorbance at 340nm at room temperature for 5min. Buffer: pH 7.3, 20 mM Tris, 50 mM NaCl, 20 µM Fe+2, 500 mM a-ketoglutarate, 2 mM ascorbic acid, 0.01% Tween-20, and 1 mM NAD+.
Description: Pseudomonas putida formaldehyde dehydrogenase (FDH) a.a. 2 - 399 (end) (GenBank Accession No. D21201) with N-terminal His-tag, MW = 42.9 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: D21201
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P46154
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ito, K., et al., J Bacteriol. 1994;176:2483-2491.
2. Tanaka, K., et al., J Mol Biol. 2002;324:519-533.
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