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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a Kinase buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA) containing 1 mM DTT using Poly-(Glu4:Tyr)substrate (0.2 mg/ml) and 20 µM ATP for 30 minutes at 30°C. The amount of ATP transferred was alculated using Kinase-Glo Plus™ reagent (Promega).
Description: Human feline sarcoma oncogene (FES), GenBank Accession # NM_002005, a.a. 2-822 (end), N-terminal His-tag and N-terminal FLAG-tag, MW=95.3 kDa, expressed in Sf9 insect cells via a baculovirus expression system
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 3 mM DTT, 2.2 mM KCl, 20% glycerol, 80 µg/ml FLAG peptide.
Genbank: NM_002005
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: P07332
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hellwig S, Smithgall TE. Front Biosci. 2011 Jun 1,17:3146-55.
2. Kanda S, Miyata Y. Front Biosci. 2011 Jan 1,16:1024-35.
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