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Encompassing Amino Acids: 447-761
Description: Human FGFR3, GenBank Accession # NM_000142, with V443M mutation, with N-terminal His-tag, a.a 447-761, MW= 37 kDa, expressed in Sf9 cells via a baculovirus expression system. Phosphorylation is <10%.
Format: Aqueous Buffer Solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.02% Tween, 20% glycerol, 3mM DTT
Genbank: NM_000142
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P22607
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Chen L. et al. Front Biosci. 2005; 296(1)10: 1961-1976.2. Mhawech. et al. Eur.J.Surg.Oncol; 2006 ;32(2):231-237.