Application Note: Anti-Fibrinogen Biotin antibody has been tested by ELISA, western blot, and immunohistochemistry. This product is assayed against 1.0 µg of Fibrinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:250,000 of the reconstitution concentration is suggested for this product.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 1:2,000 - 1:10,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:500 - 1:2,500
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Fibrinogen [Human Plasma]
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Fibrinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma]. Cross reactivity against Fibrinogen from other sources is unknown.
Background: Anti-Fibrinogen antibody has a dual function: yielding monomers that polymerize into fibrin and as a main protein of the blood coagulation system, it also functions as a cofactor in platelet aggregation. Fibrinogen clotting underlies pathogenesis of thromboembolism, MI, and thrombus formation. Fibrinogen activation is a major factor in the mechanism which causes inflammation, tumor growth and various additional diseases. These characteristics make Anti-Fibrinogen ideal for investigators interested in the fields of Cardiovascular or Metabolism research.
Low Endotoxin: No
Other: User Optimized
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