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Encompassing Amino Acids: 208-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinase and member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo (1). Overexpression of FRK in beta-cells from the pancreas increases the susceptibility of these cells to beta-cell-toxic events (hallmark of Type I diabetes)(2).
Description: Recombinant human FRK (208-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_002031.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_002031
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P42685
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Craven, R J. et al. Cancer Res. 1995 Sep 15,55(18):3969-72.
2. Welsh, M. et al. Biochem J. 2004 Aug 15,382(Pt 1):261-8.
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