Application Note: This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing over expressed protein. A 1:100 to 1:500 dilution shows reactivity with a band at 19.2kDa corresponding to mouse GADD45 gamma. Use 10 µL for immunoprecipitation.
Concentration Value: 85 mg/mL
ELISA Dilution: 1:10,000 - 1:50,000
Immunoprecipitation Dilution: Use 10 µL
Western Blot Dilution: 1:100 - 1:500
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 142-159 of Mouse GADD45 gamma. Swiss Prot accession number Q9Z111. This sequence represents the C-terminal end of the protein. Mouse and rat GADD45 g show 100% sequence homology. A single amino acid change is found in the human sequence (see below).
Physical State: Liquid (sterile filtered)
Purity and Specificity: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination. Reactivity occurs against mouse PI3K p110d subunit. Cross reactivity is expected against the human protein as the sequence of the immunogen is 100% identical in both human and mouse. Cross reactivity is also expected against rat PI3K p110d.
Background: GADD45 gamma (Growth arrest and DNA-damage-inducible protein also known as cytokine responsive protein CR6) is derived from a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45 gamma is highly expressed in placenta.
Low Endotoxin: No
Other: User Optimized
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