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Encompassing Amino Acids: full length
Applications: Useful as a quantitative control for GFPexpression studies using GFP reportervectors. It can also be used as a standard onprotein gels and Westernimmunoblots or forcalibration of fluorometers, flow cytometersand FACS machines.
Background: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Description: Recombinant Jellyfish (Aequorea victoria) green fluorescent protein, full length with C-terminal His-tag, MW= 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P42212
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chudakov DM, et al. Physiol Rev. 2010Jul;90(3):1103-63.
2. Gerdes HH, Kaether C. FEBS Lett. 1996Jun 24;389(1):44-7.
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