Application Note: Anti-Glucose Oxidase Antibody has been assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Rabbit) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for Anti-Glucose Oxidase Antibody.
Concentration Value: 90 mg/mL
ELISA Dilution: 1:5,000 - 1:25,000
Western Blot Dilution: 1:500 - 1:3,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Glucose Oxidase [Aspergillus niger]
Physical State: Lyophilized
Purity and Specificity: Anti-Glucose Oxidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Background: Glucose Oxidase, first described in Aspergillus niger), catalyzes the oxidation of glucose into gluconic acid whilst in an aerobic environment. Glucose Oxidase is one of the most widely used biomarkers due to the large enthalpy change observed when it oxidizes glucose. Glucose oxidase can be used as a label in immunoassays when attached to antibodies and for staining tissues. In respect to glucose oxidases' ability to stain tissues, it is most commonly used to determine the levels of glucose in blood, serum, and plasma, as part of colorimetric assays. Anti-Glucose Oxidase Antibody is ideal for investigators in Immunology, Molecular Biology, and Hematology Research.
Low Endotoxin: No
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