Application Note: GM-CSF protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-GM-CSF in immunological assays.
Concentration Value: 1 mg/mL
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Biological Activity: Human GM-CSF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent induction of human TF-1 cell proliferation, is 0.094 ng/ml.Endotoxin Level: Measured by LAL is <0.01ng/µg or <0.1EU/µg.
Physical State: Lyophilized
Purity and Specificity: Recombinant protein corresponding to amino acids 18 to 144 of mature human GM-CSF with addition of methionine on the N-terminus. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by analytical RP-HPLC, each against a known standard.
Background: Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL -6 , IL-7 , EPO and the Prolactin receptors. Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular weight of 14.5 kDa.
Low Endotoxin: Yes
Other: User Optimized
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