Clone: Polyclonal
Background: The antiserum is reacting with to the Fc subunits of IgM. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected..This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified IgM prepared from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Tetramethylrhodamine isothiocyanate-conjugated F(ab’)2 fragments of polyclonal Goat IgG antibodies to Human IgM, Fc specific
Formulation: TRITC-coupled goat F(abÕ)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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