Clone: Polyclonal
Background: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum containsantibodies to subclass-specific determinants as well as to determinants shared by two or more subclassesof IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserumconcentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained withIgA, IgM and IgG/Fab fragments or any other serum protein. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG, Fc specific.
Formulation: TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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