Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris/HCl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl
2, and 0.1 mg/ml BSA, 20 µM BPSHDAC substrate 3 (Catalog #50037),and HDAC1. HDAC reaction was donefor 30 min at 37°C followed by treatmentwith 50 µl of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Description: Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal FLAG and C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol
Genbank: NM_004964
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag, C-terminal His-tag
Uniprot: Q13547
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rikimaru,T. et al., Oncology 72 (1-2), 69-74 (2007).
2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).Application References:
1. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases (2015)
2. A chimeric SERM-histone deacetylase inhibitor approach to breast cancer therapy (2014)
3. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis (2014)
4. Design, synthesis and evaluation of novel HDAC inhibitors as potential antitumor agents (2014)
5. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
6.
Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
7. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
8. Meyners, C., et al. Anal Biochem. 2014 Sep 1;460:39-46. doi: 10.1016/j.ab.2014.05.014. Kinetic method for the large-scale analysis of the binding mechanism of histone deacetylase inhibitors (2014)
9. Selective HDAC Inhibition for the Disruption of Latent HIV-1 Infection (2014)
10. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
11. Meyners, C., et al. Mol Recognit. 2014 Mar;27(3):160-72. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014)
12. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
13. Class I HDAC imaging using [ (3)H]CI-994 autoradiography (2013)
14. Fass, D.M., et al. Neuropharmacology. 2013 Jan; 64: 81–96. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
15. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
16.Baud, MGJ, et al. ChemMedChem. 2013 Jan;8(1):149-56. doi: 10.1002/cmdc.201200450. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
17. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹¸F-FAHA (2013)
18. Marek, M., et al. PLoS Pathog. 2013;9(9):e1003645. doi: 10.1371/journal.ppat.1003645. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni (2013)
19. Baud, MGJ, et al. Beilstein J Org Chem. 2013;9:81-8. doi: 10.3762/bjoc.9.11. Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors (2013)
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