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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC8 (3.1 - 200 ng). Incubation condition: 30 min at 37°C, followed byHDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Description: Human HDAC8 , GenBank Accession No. NM_018486, full length with C-terminal His-tag, MW= 46.4 kDa, expressed in a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, and 10% glycerol.
Genbank: NM_018486
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q9BY41
Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme