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Encompassing Amino Acids: 23-652
Assay Conditions: This protein was tested by ELISA. A 96-well plate was coated with HER2, Avi-His-Tag Recombinant overnight at 4°C (50 µl/well at a concentration of 100 ng/reaction in PBS). The next day the plate was washed 3 times with PBST and blocked using 200 µl of Blocking Buffer 3 (#79743) for 2 hours at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of purified Anti-HER2, Sortase-His-tag Antibody (#101689), serially diluted in 1x PP-02 Buffer, was added for 1 hour at RT. After washing, the plate was incubated for 60 minutes with anti-Fc-HRP-labeled antibody 1:1000 in Blocking Buffer 3 (50 µl/well) and washed again. Finally, 100 µl of ECL substrate was added to each well and plate was read immediately in a luminometer or microtiter-plate reader capable of reading chemiluminescence.
Concentration: 0.14 mg/ml
Description: Recombinant human HER2 (human epidermal growth factor receptor 2, also known as ErbB2), encompassing amino acids 23-652 corresponding to the extracellular domain. This construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). This protein was affinity purified. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_004448.4
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-His-tag
Uniprot: P04626
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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