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Encompassing Amino Acids: 708-993
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The enzyme reaction was carried out at 37°C for 20 minutes in a buffer containing 20 mM Tris-HCl, pH 7.4, 10 mM of MgCl2, 1 mM of MnCl2, 1 mM of DTT, 1 mM of ATP, and 1 µg/ml poly(Glu4-Tyr) peptide substrate.
Description: Human HER4 kinase (ERBB4) (GenBank Accession No. NM_005235), a.a. 708-993, with N-terminal GST-tag, MW=59.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
Genbank: NM_005235
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q15303
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Strunk, K.E. et al., Cancer Res. 67 (14), 6582- 6590 (2007).
2. Law, A.J. et al., Hum. Mol. Genet. 16 (2), 129-141 (2007).