Application Note: WB: 1:500-1:3000. IHC-Wm: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 15
Positive Control: 3dpf zebrafish embryos (treated w/wo UV irradiation) , 3dpf zebrafish embryos
Form: Liquid
Buffer (with preservative): PBS, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Uniprot ID: Q7ZUY3
Antigen Species: Zebrafish
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Histone H2A.X. The exact sequence is proprietary.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: H2A histone family, member X
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