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Applications: A perfect substrate for histone methyltransferases.
Description: Human recombinant histone tetramer consisting of 2 molecules each of histones H3 (a.a. 2-136(end)) and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Global MW = 57 kDa.
Format: Aqueous buffer solution
Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
Genbank: NM_033445, NM_003528, NM_003532, and NM_003548
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P68431
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46
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