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Encompassing Amino Acids: 2-136(end)
Applications: A perfect substrate for histone demethylases.
Description: Human recombinant histone H3 (a.a. 1-136(end)) with K27C, C97A, and C111A mutations, and an N-terminal His-tag, expressed in an E. coli expression system. GenBank Accession No. NM_003532. The protein has been alkylated to produce the lysine analog, trimethyl aminoethylcysteine. MW = 16 kDa.
Format: Aqueous buffer solution
Formulation: PBS (8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl), 20% glycerol.
Genbank: NM_003532
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P68431
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.
2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46