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Encompassing Amino Acids: 2-215
Applications: Useful as a positive control for Western blot studies.
Description: Human HMGB1 also known as high-mobility group protein 1, and amphoterin, GenBank Accession No. NM_002128, a.a. 2-215, with C-terminal Avi-His-Tag, and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTagTM technology. MW = 25 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_002128
Purity: ≥90%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: C-terminal Avi-His-Tag™, Biotin-labeled
Uniprot: P09429
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scaffidi, P., et al., Nature. 2002; 418: 191-195.2. Lotze, M., et al., Nature. 2005; 5: 331-342.
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