Application Note: Human Collagen Type III purified protein standard has been tested in SDS-Page and western blot and is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Concentration Value: 1.0 mg/ml
ELISA Dilution: 1:10,000
Immunohistochemistry Dilution: 1:100-1:500
Immunoprecipitation Dilution: 1:100-1:500
Western Blot Dilution: 1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with Anti-Collagen Type III. Reaction with Rockland’s Anti-Collagen I, II, IV, V or VI is negligible.
Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Collagen type III, a fibrous scleroprotein found in bone, cartilage, tendon, bone marrow stroma and other connective tissue, yields gelatin on boiling. Collagen Type III scleroprotein is a simple protein found in horny and cartilaginous tissues and in the lens of the eye.
Low Endotoxin: No
Other: User Optimized
English
Deutsch
