Species: Human
Product Description: Epidermal Growth Factor (EGF) is a 53-a.a. peptide cleaved from a 1207 a.a. transmembrane glycoprotein precursor. Its binding to the EGF receptor (EGFR) affects cell proliferation and differentiation via downstream effectors.
Application Note: The determined ED50 is ≤ 0.1 ng/mL, which corresponds to a specific activity of ≥ 1 x 107 units/mg and is comparable to similar commercial products. The biological activity of this recombinant human EGF protein was analyzed using the BALB/c 3T3 Clone A31 mouse embryonic fibroblast cell proliferation assay (Rubin, J.S. et al. (1991)).
Form: Lyophilized powder
Buffer (with preservative): Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from 50mM PBS, 100mM NaCl, 5% Trehalose, no Preservative.
Concentration:
Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
Uniprot ID: P01133
Region Sequence: Mature human EGF protein ( Asn971~Arg1023 of NP_001954.2)
ExpressionSystem: E. Coli
Conjugation: Unconjugated
Full Name: epidermal growth factor
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