Application Note: Human Transferrin Horseradish Peroxidase (HRP) has been tested in dot blot and is a suitable protein for use as a control reagent in both Western Blotting and ELISA experiments.
Concentration Value: 1.0 mg/mL
ELISA Dilution: 4,000 - 10,000
Immunohistochemistry Dilution: 200 - 2,000
Western Blot Dilution: 2,000 - 5,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Lyophilized
Purity and Specificity: Human Transferrin Peroxidase conjugated was prepared from normal serum by a multi-step process including selective precipitation and tandem chromatography followed by extensive dialysis against the buffer stated above. Human Transferrin Peroxidase conjugated was assayed by immunoelectrophoresis and resulted in a single precipitin arc against anti-Peroxidase, anti-Human Transferrin and anti-Human Serum.
Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
Low Endotoxin: No
Other: User Optimized
English
Deutsch
