Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for screening small molecular inhibitors for drug discovery and HTS applications.
Background: Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH2, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH2(R140Q) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH2(R140Q) mutation in various cancer types, including as glioma, chondrosarcoma, AML as well as small percentage of prostate, lung and colon cancers.
Description: The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The IDH2(R140Q) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH2(R140Q) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Wang, F. et al. Science 340:622-626 (2013).
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